Cell-Based Screening and Profiling Services.Cell Culture and Transient Transfection.Transient Transfection Vector Construction.Induced Expression Stable Cell Line Construction Service.Gene Knock-In Cell Line Construction Service.Precisely Point-to-point Integrated Cell Line Construction.Gene Knockdown Cell Line Construction Service.Gene Knockout Cell Line Construction Service.Gene Silencing Cell Line Construction Service.Gene Overexpression Cell Line Construction Service.Cell Cryopreservation and Cell Banking Services.Tumor Cell Isolation and Culture Services.Immune Cell Isolation and Culture Services.Blood Cell Isolation and Culture Services.Stem Cell Isolation and Culture Services.Primary Cell Isolation and Culture Services.Basic and Large-Scale Cell Culture Services.Molecular epidemiology of Pseudomonas aeruginosa-urinary tract infections in paraplegic patients. Worlitzsch D, Wolz C, Botzenhart K, Hansis M, Burgdörfer H, Ogle JW, Döring G.Pseudomonas aeruginosa cross-colonization and persistence in patients with cystic fibrosis. Wolz C, Kiosz G, Ogle JW, Vasil ML, Schaad U, Botzenhart K, Döring G.Nosocomial acquisition of Pseudomonas aeruginosa by cystic fibrosis patients. Tümmler B, Koopmann U, Grothues D, Weissbrodt H, Steinkamp G, von der Hardt H.Use of a pilin gene probe to study molecular epidemiology of Pseudomonas aeruginosa. Speert DP, Campbell ME, Farmer SW, Volpel K, Joffe AM, Paranchych W.Detection of specific sequences among DNA fragments separated by gel electrophoresis. Biotinylated DNA probes for exotoxin A and pilin genes in the differentiation of Pseudomonas aeruginosa strains. Person-to-person transmission of Pseudomonas cepacia between patients with cystic fibrosis. LiPuma JJ, Dasen SE, Nielson DW, Stern RC, Stull TL.Interactions of Pseudomonas aeruginosa proteases with the cells of the immune system.
Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains.
Southern hybridization analysis full#
Full textįull text is available as a scanned copy of the original print version. The patterns of hybridization to the elastase and algD gene probes were highly conserved in all isolates, therefore, these DNA fragments are not useful in discriminating strains, in contrast to the exotoxin A gene probe. aeruginosa were used in Southern hybridization analysis. DNA fragments from the alginate biosynthesis gene complex, the exotoxin A gene, and the elastase gene of P. aeruginosa DNAs from cystic fibrosis isolates however, sequential isolates obtained from individual patients showed very little variation over an 8-year period. There was marked diversity of restriction enzyme analysis patterns among P.
aeruginosa isolates from patients with cystic fibrosis were examined by restriction enzyme analysis and Southern hybridization. Conventional typing schemes for Pseudomonas aeruginosa may not discriminate strains.
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